Educação
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05/85: Graduação no Quirinus-Gymnasium Neuss (Alemanha).
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04/88 - 10/92: Graduação em biologia pela Universidade Heinrich Heine, Düsseldorf, Alemanha.
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11/92 - 10/96: Estudo principal em biologia pela Universidade Heinrich Heine, Düsseldorf, Alemanha.
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10/95 - 10/96: Apresentação da Tese: "Identificação e caracterização de transcritos contendo repetição B1 no baço de camundongos B10 imunes a P. chabaudi". Instituto de Parasitologia Molecular, Universidade Heinrich-Heine Düsseldorf, Alemanha.
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10/96: Exame de Graduação
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04/97 - 06/00: Tese de doutorado: "Alterações na metilação em células tumorais urológicas e efeito da superexpressão de DNA- (citosina-5) - metil-transferase 1 (DNMT1) em linhas de células tumorais". Laboratório de pesquisa da clínica urológica Heinrich Heine – Universidade Düsseldorf, Alemanha.
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07/00: Exame de doutorado (Magna cum laude).
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07/12: Habilitação e Venia Legendi para Medicina Molecular na Faculdade de Medicina da Universidade Heinrich-Heine, Düsseldorf, Alemanha.
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07/19: Nomeação como professor extraordinário de medicina molecular na faculdade de medicina da Universidade Heinrich Heine, Düsseldorf, Alemanha.
Prática em Pesquisa
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10/95 - 10/96: Preparação de tese e trabalho de pesquisa no grupo de trabalho do Prof. F. Wunderlich, no Instituto de Parasitologia Molecular da Universidade Heinrich-Heine, Düsseldorf, Alemanha.
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04/97 - 06/00: Trabalho de pesquisa como parte da tese de doutorado no grupo de trabalho do Prof. W. A. Schulz, no laboratório de pesquisa da clínica urológica do Prof. R. Ackermann, Universidade Heinrich-Heine, Alemanha.
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12/00 - 12/07: Pós-doutorado no grupo de pesquisa júnior "Imunidade Natural" do PD Dr. Markus Uhrberg, do Instituto de Diagnóstico de Transplantes e Terapêutica Celular, pelo Prof. P. Wernet, Universidade Heinrich-Heine, Düsseldorf, Alemanha.
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(Desde) 01/08: Chefe do Laboratório Central de Pesquisa em Epigenética do Instituto de Diagnóstico de Transplantes e Terapêutica Celular (Diretor de Comunicações, Dr. J. Fischer).
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07/12: Habilitação em medicina molecular na Universidade Heinrich-Heine, Düsseldorf, Alemanha.
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07/19: Título de “Professor”, Universidade Heinrich-Heine, Düsseldorf, Alemanha, em 27.03.2020, Prof. Priv.-Doz. Dr. rer. nat. Simeon Santourlidis.
Publications:
1. Basic Hallmarks of Urothelial Cancer Unleashed in Primary Uroepithelium by Interference with the Epigenetic Master Regulator ODC1.
Erichsen L, Seifert HH, Schulz WA, Hoffmann MJ, Niegisch G, Araúzo-Bravo MJ, Bendhack ML, Poyet C, Hermanns T, Beermann A, Hassan M, Theis L, Mahmood W, Santourlidis S.
Sci Rep. 2020 Mar 2;10(1):3808.
2. Epigenetics Meets CRISPR/Cas to Fight Cancer Simeon Santourlidis J Cancer Res Therap Oncol. 2020 March 03, 8: 1-5.
3. Evaluation of nanoselenium and nanogold activities against murine intestinal schistosomiasis. Dkhil MA, Khalil MF, Diab MSM, Bauomy AA, Santourlidis S., Al-Shaebi EM, Al-Quraishy S.
Saudi J Biol Sci. 2019 Nov;26(7):1468-1472.
4. Tumor necrosis factor-α triggers opposing signals in head and neck squamous cell carcinoma and induces apoptosis via mitochondrial- and non-mitochondrial-dependent pathways.
Selimovic D, Wahl RU, Ruiz E, Aslam R, Flanagan TW, Hassan SY, Santourlidis S., Haikel Y, Friedlander P, Megahed M, Kandil E, Hassan M.
Int J Oncol. 2019 Dec;55(6):1324-1338.
5. Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging.
Erichsen L, Beermann A, Arauzo-Bravo MJ, Hassan M, Dkhil MA, Al-Quraishy S, Hafiz TA, Fischer JC, Santourlidis S..
Saudi J Biol Sci. 2018 Sep;25(6):1220-1226.
6. Author Correction: Aberrant methylated key genes of methyl group metabolism within the molecular etiology of urothelial carcinogenesis.
Erichsen L, Ghanjati F, Beermann A, Poyet C, Hermanns T, Schulz WA, Seifert HH, Wild PJ, Buser L, Kröning A, Braunstein S, Anlauf M, Jankowiak S, Hassan M, Bendhack ML, Araúzo-Bravo MJ, Santourlidis S.
Sci Rep. 2018 Apr 11;8(1):6051.
7. Aberrant methylated key genes of methyl group metabolism within the molecular etiology of urothelial carcinogenesis.
Erichsen L, Ghanjati F, Beermann A, Poyet C, Hermanns T, Schulz WA, Seifert HH, Wild PJ, Buser L, Kröning A, Braunstein S, Anlauf M, Jankowiak S, Hassan M, Bendhack ML, Araúzo-Bravo MJ, Santourlidis S.
Sci Rep. 2018 Feb 22;8(1):3477.
8. Protective vaccination and blood-stage malaria modify DNA methylation of gene promoters in the liver of Balb/c mice.
Al-Quraishy S, Dkhil MA, Abdel-Baki AS, Ghanjati F, Erichsen L, Santourlidis S., Wunderlich F, Araúzo-Bravo MJ. Parasitol Res. 2017 May;116(5):1463-1477.
9. Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways.
El Jamal SM, Taylor EB, Abd Elmageed ZY, Alamodi AA, Selimovic D, Alkhateeb A, Hannig M, Hassan SY, Santourlidis S., Friedlander PL, Haikel Y, Vijaykumar S, Kandil E, Hassan M.
Cell Div. 2016 Aug 2;11:11.
10. IDLN-MSP: Idiolocal normalization of real-time methylation-specific PCR for genetic imbalanced DNA specimens.
Santourlidis S., Ghanjati F, Beermann A, Hermanns T, Poyet C.
Biotechniques. 2016 Feb 1;60(2):84-7.
11. Effect on Multipotency and Phenotypic Transition of Unrestricted Somatic Stem Cells from Human Umbilical Cord Blood after Treatment with Epigenetic Agents.
Ghanjati F, Santourlidis S.
Stem Cells Int. 2016;2016:7643218.
12. Age-Related Increase of EED Expression in Early Hematopoietic Progenitor Cells is Associated with Global Increase of the Histone Modification H3K27me3.
Graffmann N, Brands J, Görgens A, Vitoriano da Conceição Castro S, Santourlidis S., Reckert A, Michele I, Ritz-Timme S, Fischer JC, Adjaye J, Kögler G, Giebel B, Uhrberg M.
Stem Cells Dev. 2015 Sep 1;24(17):2018-31.
13. Corrigendum to "Bortezomib/proteasome inhibitor triggers both apoptosis and autophagydependent pathways in melanoma cells". [Cell Signal. 25(1)Jan 2013 308-18].
Selimovic D, Porzig BB, El-Khattouti A, Badura HE, Ahmad M, Ghanjati F, Santourlidis S., Haikel Y, Hassan M. Cell Signal. 2015 Feb 6.
14. Erythroid differentiation of human induced pluripotent stem cells is independent of donor cell type of origin.
Dorn I, Klich K, Arauzo-Bravo MJ, Radstaak M, Santourlidis S., Ghanjati F, Radke TF, Psathaki OE, Hargus G, Kramer J, Einhaus M, Kim JB, Kögler G, Wernet P, Schöler HR, Schlenke P, Zaehres H.
Haematologica. 2015 Jan;100(1):32-41.
15. Origin-dependent neural cell identities in differentiated human iPSCs in vitro and after transplantation into the mouse brain.
Hargus G, Ehrlich M, Araúzo-Bravo MJ, Hemmer K, Hallmann AL, Reinhardt P, Kim KP, Adachi K, Santourlidis S., Ghanjati F, Fauser M, Ossig C, Storch A, Kim JB, Schwamborn JC, Sterneckert J, Schöler HR, Kuhlmann T, Zaehres
H.
Cell Rep. 2014 Sep 25;8(6):1697-1703.
16. Unreserved application of epigenetic methods to define differences of DNA methylation between urinary cellular and cell-free DNA.
Ghanjati F, Beermann A, Hermanns T, Poyet C, Araúzo-Bravo MJ, Seifert HH, Schmidtpeter M, Goering W, Sorg R, Wernet P, Santourlidis S.
Cancer Biomark. 2014;14(5):295-302.
17. Genome-wide screening identifies Plasmodium chabaudi-induced modifications of DNA methylation status of Tlr1 and Tlr6 gene promoters in liver, but not spleen, of female C57BL/6 mice.
Al-Quraishy S, Dkhil MA, Abdel-Baki AA, Delic D, Santourlidis S., Wunderlich F.
Parasitol Res. 2013 Nov;112(11):3757-70.
18. Vinblastine-induced apoptosis of melanoma cells is mediated by Ras homologous A protein (Rho A) via mitochondrial and non-mitochondrial-dependent mechanisms.
Selimovic D, Badura HE, El-Khattouti A, Soell M, Porzig BB, Spernger A, Ghanjati F, Santourlidis S., Haikel Y, Hassan M.
Apoptosis. 2013 Aug;18(8):980-97.
19. Bortezomib/proteasome inhibitor triggers both apoptosis and autophagy-dependent pathways in melanoma cells.
Selimovic D, Porzig BB, El-Khattouti A, Badura HE, Ahmad M, Ghanjati F, Santourlidis S., Haikel Y, Hassan M. Cell Signal. 2013 Jan;25(1):308-18. Erratum in: Cell Signal. 2015 Mar;27(3):727. Cell Signal. 2015
May;27(5):1019-1020.
20. Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions Agnes Beermann, Foued Ghanjati, Thomas Hermanns, Cedric Poyet, Joana Pereira, Johannes Fischer, Peter Wernet and Simeon Santourlidis.
The Open Biomarkers Journal, 2011, 4, 15-17.
21. Unrestricted somatic stem cells (USSC) from human umbilical cord blood display uncommitted epigenetic signatures of the major stem cell pluripotency genes.
Santourlidis S., Wernet P, Ghanjati F, Graffmann N, Springer J, Kriegs C, Zhao X, Brands J, Araúzo-Bravo MJ, Neves R, Koegler G, Uhrberg M.
Stem Cell Res. 2011 Jan;6(1):60-9.
22. Role of DNA methylation in miR-200c/141 cluster silencing in invasive breast cancer cells.
Neves R, Scheel C, Weinhold S, Honisch E, Iwaniuk KM, Trompeter HI, Niederacher D, Wernet P, Santourlidis S., Uhrberg M.
BMC Res Notes. 2010 Aug 3;3:219.
23. Induction of pluripotency in human cord blood unrestricted somatic stem cells.
Zaehres H, Kögler G, Arauzo-Bravo MJ, Bleidissel M, Santourlidis S., Weinhold S, Greber B, Kim JB, Buchheiser A, Liedtke S, Eilken HM, Graffmann N, Zhao X, Meyer J, Reinhardt P, Burr B, Waclawczyk S, Ortmeier C, Uhrberg M, Schöler HR, Cantz T, Wernet P.
Exp Hematol. 2010 Sep;38(9):809-18, 818.e1-2.
24. The HOX Code as a "biological fingerprint" to distinguish functionally distinct stem cell populations derived from cord blood.
Liedtke S, Buchheiser A, Bosch J, Bosse F, Kruse F, Zhao X, Santourlidis S., Kögler G.
Stem Cell Res. 2010 Jul;5(1):40-50.
25. Induction of indoleamine 2, 3-dioxygenase by death receptor activation contributes to apoptosis of melanoma cells via mitochondrial damage-dependent ROS accumulation.
Cetindere T, Nambiar S, Santourlidis S, Essmann F, Hassan M.
Cell Signal. 2010 Feb;22(2):197-211.
26. Lineage-specific transition of histone signatures in the killer cell Ig-like receptor locus from hematopoietic progenitor to NK cells.
Santourlidis S., Graffmann N, Christ J, Uhrberg M.
J Immunol. 2008 Jan 1;180(1):418-25.
27. Nucleolin regulates gene expression in CD34-positive hematopoietic cells.
Grinstein E, Du Y, Santourlidis S, Christ J, Uhrberg M, Wernet P. J Biol Chem. 2007 Apr 27;282(17):12439-49.
28. Nucleolin activates expression of CD34 and Bcl-2in CD34-positive hematopoietic cells.
Grinstein E, Santourlidis S., Fischer J, Uhrberg M, Wernet P. J Stem Cells Regen Med. 2007 May 16;2(1):18-9.
29. Direct and quantitative analysis of chromatin accessibility by MIRECAL--a Micrococcus nuclease/real-time PCR chromatin accessibility assay with locus specificity.
Graffmann N, Santourlidis S., Christ J, Wernet P, Uhrberg M.
Anal Biochem. 2006 Jul 15;354(2):308-10. Epub 2006 Apr 19.
30. Molecular characterization of KIR3DL3.
Trundley AE, Hiby SE, Chang C, Sharkey AM, Santourlidis S., Uhrberg M, Trowsdale J, Moffett A. Immunogenetics. 2006 Jan;57(12):904-16.
31. Three structurally and functionally divergent kinds of promoters regulate expression of clonally distributed killer cell Ig-like receptors (KIR), of KIR2DL4, and of KIR3DL3.
Trompeter HI, Gómez-Lozano N, Santourlidis S., Eisermann B, Wernet P, Vilches C, Uhrberg M. J Immunol. 2005 Apr 1;174(7):4135-43.
32. Suppression of clonogenicity by mammalian Dnmt1 mediated by the PCNA-binding domain. Santourlidis S., Kimura F, Fischer J, Schulz WA.
Biochem Cell Biol. 2004 Oct;82(5):589-96.
33. Downregulation of CD44v6 in colorectal carcinomas is associated with hypermethylation of the CD44 promoter region.
Stallmach A, Wittig BM, Kremp K, Goebel R, Santourlidis S., Zeitz M, Menges M, Raedle J, Zeuzem S, Schulz WA. Exp Mol Pathol. 2003 Jun;74(3):262-6.
34. Decrease of DNA methyltransferase 1 expression relative to cell proliferation in transitional cell carcinoma.
Kimura F, Seifert HH, Florl AR, Santourlidis S., Steinhoff C, Swiatkowski S, Mahotka C, Gerharz CD, Schulz WA. Int J Cancer. 2003 May 1;104(5):568-78.
35. Crucial role of DNA methylation in determination of clonally distributed killer cell Ig-like receptor expression patterns in NK cells.
Santourlidis S., Trompeter HI, Weinhold S, Eisermann B, Meyer KL, Wernet P, Uhrberg M.
J Immunol. 2002 Oct 15;169(8):4253-61.
36. Genomewide DNA hypomethylation is associated with alterations on chromosome 8 in prostate carcinoma.
Schulz WA, Elo JP, Florl AR, Pennanen S, Santourlidis S., Engers R, Buchardt M, Seifert HH, Visakorpi T. Genes Chromosomes Cancer. 2002 Sep;35(1):58-65.
37. Refined mapping of allele loss at chromosome 10q23-26 in prostate cancer.
Leube B, Drechsler M, Mühlmann K, Schäfer R, Schulz WA, Santourlidis S., Anastasiadis A, Ackermann R, Visakorpi T, Müller W, Royer-Pokora B.
Prostate. 2002 Feb 15;50(3):135-44.
38. Hypermethylation of the tumor necrosis factor receptor superfamily 6 (APT1, Fas, CD95/Apo-1) gene promoter at rel/nuclear factor kappaB sites in prostatic carcinoma.
Santourlidis S., Warskulat U, Florl AR, Maas S, Pulte T, Fischer J, Müller W, Schulz WA.
Mol Carcinog. 2001 Sep;32(1):36-43.
39. High frequency of alterations in DNA methylation in adenocarcinoma of the prostate. Santourlidis S., Florl A, Ackermann R, Wirtz HC, Schulz WA.
Prostate 1999 May 15;39(3):166-74.
40. Identification of the physiological promoter for spinocerebellar ataxia 2 gene reveals a CpG island for promoter activity situated into the exon 1 of this gene and provides data about the origin of the nonmethylated state of these types of islands.
Aguiar J, Santourlidis S., Nowok J, Alexander C, Rudnicki D, Gispert S, Schulz W, Auburger G.
Biochem Biophys Res Commun. 1999 Jan 19;254(2):315-8.